Vitamins (Group B vitamins, choline, folic acid, glycol, nicotine)

Eagle's MEM contains only water-soluble vitamins (group B vitamins, choline, folic acid, glycol, nicotine, but does not include biotin), including serum-free medium M199 CMRL1066 (from M199) and RPMI1640 contain p-ammonia Benzoic acid (PABA). Although LHC-9 contains vitamin A and MCDB110 contains vitamin E, only M199 contains all fat-soluble vitamins (A, D, E, K), and certain vitamins (such as gall (Alkali and nicotine) concentration has increased. The amount of vitamins added is limited, for example, folic acid will precipitate in a concentrated storage solution. Maximum cell density. As with certain amino acids, the vitamin requirements are based on experience, and are usually related to the culture medium initially used during the establishment of a cell line. For example, Fisher's medium contains high concentrations of folic acid because of L5178Y's dependence on folic acid was used in the development of this medium.


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